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Some enhancer is located in the upstream of the promoter, some is located in the downstream, so insulator effect does not depend on insulator with the relative position of the promoter. Therefore, the insulator effect of directional reason has not really clear.

At present has found that there are two loci to trans activation way affect the function of the insulator. Gene S2J (Hw) coded nucleoprotein recognition insulator, insulator with its combination have insulation effect.

When the gene mutation, although y loci insert the insulator, but lost the insulation effect, y in all organizations in all expression. Another loci is mod (MDG, this gene mutations, its effect as it happens and Su (Hw) instead, namely the mutant have strengthened insulation effect, make insulator insulation effect no longer have directional and

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